Ping Li学术报告

来源：浙江大学化学系发布时间：2011-05-26 7176

题目 Mechanistic Studies of Polyhydroxybutyrate
(PHB) Biosynthesis

报告人 Ping Li
Dept. Chem., Kansas State University,

简介
PHB synthases catalyze the conversion of 3-hydroxybutyryl coenzyme A (HBCoA) to PHB with a molecular mass of 1.5 MDa. The polymerization involves covalent catalysis with a Cys. Two mechanisms for elongation have been proposed. The first involves an active site composed of two monomers in which the growing hydroxybutyrate (HB) chain alternates between Cys on each monomer. The second involves covalently attached intermediates and noncovalent (HB)nCoA intermediates. Three strategies have been developed to distinguish between these models. Our results support the mechanism involving covalently and noncovalently bound intermediates. In addition, a loading site is proposed for the priming process. Understanding the mechanism of polymerization will help us engineer PHB synthases to produce biodegradable polymers in a controlled fashion.

2000-2004 Graduate Research Assistant, Duke University
2005-2006 Postdoctoral Associate, Duke
2006-2011 Postdoctoral Fellow, Massachusetts Institute of Technology 2011- Present Assistant Professor, Chemistry, Kansas State University
1. Li, P.; Chakraborty, S and Stubbe, J. “Detection of Covalent and Non-covalent Intermediates in the Polymerization Reaction Catalyzed by a C149S Class III Polyhydroxybutyrate Synthase.” Biochemistry, 2009, 9202-9211
2. Li, P.; Sergueeva, Z. A. and Shaw, B. R. “Nucleoside and Oligonucleoside Boranophosphates: Chemistry and Properties.” Chem. Rev. 2007, 4746-4796.
3. Li, P.; Xu, Z.; Liu, H. Y.; Wennefors, C. K.; Dobrikov, M.; Ludwig, J.; Shaw, B. R. “Synthesis of-P-Modified Nucleoside Diphosphates with Ethylenediamine.” J. Am. Chem. Soc. 2005, 16782-16783

2011年05月30日 14:00 教八-515

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